Thesis Project Form
Title (tentative): Development of a microfluidic device for the encapsulation of cells in hydrogel microbeads.| Thesis advisor(s): Pastorino Laura | E-mail: |
| Address: Via Opera Pia 13 | Phone: (+39) 010 33 56547 |
Description
Motivation and application domain
Development and characterization of a device for cell encapsulation for applications in tissue engineering.
General objectives and main activities
The device will be developed by 3D printing techniques.
Biopolymeric microbeads will be generated by the microfluidic device and the ability of encapsulating cell will be evaluated. The biopolymers to be used will be polysaccharides, such as alginate or chitosan, or modified proteins, such as GELMA. The microbeads will be characterized by optical and confocal microscopy and in vitro tested using immortalized cell lines.
Biopolymeric microbeads will be generated by the microfluidic device and the ability of encapsulating cell will be evaluated. The biopolymers to be used will be polysaccharides, such as alginate or chitosan, or modified proteins, such as GELMA. The microbeads will be characterized by optical and confocal microscopy and in vitro tested using immortalized cell lines.
Training Objectives (technical/analytical tools, experimental methodologies)
To gain practical experience in the development, engineerization and characterization of microfluidic devices for the encapsulation of cells to be used in tissue engineering applications.
Place(s) where the thesis work will be carried out: DIBRIS, University of Genova
Additional information
Maximum number of students: 1