Thesis Project Form
Title (tentative): Murine-derived neuronal assembloids: characterization of mechanical, topological and electrophysiological properties| Thesis advisor(s): Massobrio Paolo, Martina Brofiga | E-mail: |
| Address: Via All'Opera Pia, 13 - 16145 Genova | Phone: (+39) 010 33 52761 |
Description
Motivation and application domain
In vitro models of the brain based on 3D structures, realistic connectivity as well as neuronal compositions are nowadays essential. For this reasons, goal of this thesis is to provide a new experimental model where neuronal spheroids coming from different brain regions are functionally coupled to generate complex structures.
General objectives and main activities
Development of a new experimental protocol to cultivate complex neuronal structures based on cortical and hippocampal neurons;
Characterization of the so achieved structures in terms of: i) geometrical properties; ii) cell composition; iii) mechanical properties; iiii) exhibited patterns of electrophysiological activity.
Characterization of the so achieved structures in terms of: i) geometrical properties; ii) cell composition; iii) mechanical properties; iiii) exhibited patterns of electrophysiological activity.
Training Objectives (technical/analytical tools, experimental methodologies)
Cell culture procedures; Microscopy techniques; MEA-based set-up; Data analysis (MATLAB-based) algorithms.
Place(s) where the thesis work will be carried out: DIBRIS
Additional information
Pre-requisite abilities/skills: Computational Neuroscience, Matlab Programming, Lab
Curriculum: Neuroengineering
Maximum number of students: 2
Financial support/scholarship: -