Thesis Project Form

The neuronal networks derived from human induced pluripotent stem cells (hiPSCs) opened new perspectives in the neuroscience and neuroengineering fields. The evaluation of electrophysiology of 3D modular neuronal networks provides rich information and a solid baseline for future exploitation of in vitro human-derived experimental models.

The goal is to investigate the electrophysiology of 3D neuronal networks derived from hiPSCs. In particular, the aim is to characterize the complexity of the model, analyzing differences between modular and non-modular systems.
The activities involve the implementation of cell culture protocols for the generation of 3D homogeneous and heterogeneous models and the maintenance of such neuronal cultures on long-term. The activities include the definition and execution of experimental protocols for the acquisition and the analysis of the electrophysiological activity.

Data acquisition platform (3Brain)
3D neuronal cultures’ generation
3D neuronal cultures’ maintenance
Software tools for electrophysiological recordings
Software tools for data analysis