Thesis Project Form
Title (tentative): In vitro electrochemical monitoring of allergic reactions| Thesis advisor(s): Raiteri Roberto, Stephan S. Keller (DTU, Denmark) | E-mail: |
| Address: Via Opera pia 11a 16145 Genova | Phone: (+39) 010 33 52762 |
Description
Motivation and application domain
Although a lot of research has been conducted to investigate the mechanisms behind allergic reactions, there is still a lack of knowledge on several fundamental phenomena related to allergy
General objectives and main activities
The goal of this project is the development of an in vitro model which could provide real-time electrochemical monitoring of degradation of mast cells triggered by allergic reactions. Carbon microelectrodes will be fabricated and mast cells will be cultured on such electrodes. The release of histamine upon mast cell activation will be studied using different electrochemical methods (cyclic voltammetry, impedance spectroscopy, amperometry)
Training Objectives (technical/analytical tools, experimental methodologies)
The student will be trained in:
- microfluidics fabrication
- electrochemical methods (cyclic voltammetry, electrochemical impedance spectroscopy, amperometry)
- cell laboratory work (cell culturing, staining, microscopy)
- general experimental practice (planning, execution, and documentation of experiments)
- microfluidics fabrication
- electrochemical methods (cyclic voltammetry, electrochemical impedance spectroscopy, amperometry)
- cell laboratory work (cell culturing, staining, microscopy)
- general experimental practice (planning, execution, and documentation of experiments)
Place(s) where the thesis work will be carried out: Department of Health Technology, Technical University of Denmark, Kongens Lyngby, Denmark
Additional information
Maximum number of students: 1