Thesis Project Form

Stem cells (SCs) are cells, with the ability, to both self-renew and to differentiate into specialized cells, in response to appropriate signals. Cell therapy, is the most successful application of the stem cells till date. In order to understand the intricate (inter-intra) stem cells (SCs) signalling mechanisms, a sophisticated substrate
modification such as micro and nano patterning of surface is required in order to understand and control cell differentiation or proliferation.

The goal of this project is to combine the effects of micro nano patterns with different extra cellular matrices (ECM) (e.g, Poly-lysine, Laminin and Matrigel) to examine changes in human neural stem cells (hNSCs) proliferation and maturation. For this purpose, flat surfaces of different materials such as plastic, glass and other polymers would be used as a comparison to premade 3D topographies for studying cells response. These experiments would be characterized using immunochemistry, electron microscopy, and electrochemical techniques.

The student will be trained in:
- Human neural stem cell culture and differentiation methods
- Immunochemistry concepts and experiments
- Electrochemical detection techniques
- Cells characterization techniques (Light and Confocal microscopy, Scanning electron microscopy)