Thesis Project Form

Two-photon microscopy is currently one of the most widespread techniques for imaging calcium indicators that are reporters of the neuronal activity both in vivo and in vitro. This tool, coupled with optogenetics, allows for all optical visualization and manipulation (inhibition/stimulation) of a set of neurons giving the chance of studying the role of particular circuits in the central nervous system of mice

The project focuses on developing a holographic setup for photostimulation, coupled with a two-photon microscope. The student needs to develop the light shaping module containing the spacial light modulator (SLM) and design the optimal optical path based on the requirements imposed by holography (diffraction). Moreover, the student will work on the Matlab code for controlling the SLM. Finally, the setup needs to be tested for comparing its performance with older holographic modules and for verifying the activation/deactivation of opsin- expressing neurons in a biological sample

The student will learn the principles of diffraction theory governing the design and projection of holograms. This mathematical background is essential for developing the Matlab code that calculates and sends the phase masks to the SLM. The student will work with optics components and lasers on a two-photon microscope understanding its unique imaging capabilities