Thesis Project Form

Hydrogel-based scaffolds for 3D neuronal networks play a pivotal role in the emulation of in-vivo neurophysiology. Mathematical modeling can provide useful information for improving in-vitro conditions of biomimetic 3D neuronal cultures.

Aim of this thesis is to build a mathematical model of oxygen and nutrients transport and consumption in hydrogel-based porous scaffolds for 3D neuronal networks development. In particular, the dependence of cell distribution and growth on related to experimental factors such as cell density and scaffold permeability will be assessed. Different structures and media conditions will be considered. The model will be solved by finite element method (FEM) using Comsol Multiphysics software. The experimental activity will be aimed at the fabrication, characterization and in-vitro application of the scaffolds in order to validate the developed models.

The student will learn how to model transport processes in porous media. Student will also gain practical experience in the design, fabrication and characterization of biopolymeric 3D porous scaffolds with different characteristics.