Thesis Project Form
Title (tentative): 3D cell cultures under dynamic perfusion| Thesis advisor(s): Pastorino Laura, Donatella Di Lisa | E-mail: |
| Address: Via Opera Pia 13 | Phone: (+39) 010 33 56547 |
Description
Motivation and application domain
Preparation, optimization and characterization of advanced 3D brain tissue cultures under dynamic perfusion
General objectives and main activities
Advanced 3D brain tissue in vitro models will be developed under dynamic cell culture conditions and characterized.
Neurons derived from human pluripotent stem cells will be used. The 3D models will be tested under different dynamic conditions and the impact on the maturation of a functional neuronal network will be characterized by immunocytochemistry and by electrophysiological recordings.
Neurons derived from human pluripotent stem cells will be used. The 3D models will be tested under different dynamic conditions and the impact on the maturation of a functional neuronal network will be characterized by immunocytochemistry and by electrophysiological recordings.
Training Objectives (technical/analytical tools, experimental methodologies)
To gain practical experience in the development, engineerization and characterization of advanced in vitro 3D brain tissue models.
Place(s) where the thesis work will be carried out: DIBRIS, University of Genova
Additional information
Maximum number of students: 1