Thesis Project Form
Title (tentative): Electroporation and nanoparticles in Gene Electro transfer| Thesis advisor(s): Pastorino Laura, Polajzer Tamara | E-mail: |
| Address: Via Opera Pia 13 | Phone: (+39) 010 33 56547 |
Description
Motivation and application domain
Investigation of the influence of nanoparticles properties on the electro-transfer of genes during electroporation.
General objectives and main activities
Explore gene electro transfer in the context of electroporation phenomenon. Influence of nanoparticles, as local enhancer of electric field will be investigated: different composition, charge, shape, size of nanoparticles will be used. Experimental work will be on cell cultures in vitro using flow cytometer, fluorescence microscope, spectrofluorometer. Obtained results will be statistically analysed using software such as Sigmaplot.
Training Objectives (technical/analytical tools, experimental methodologies)
To gain practical experience in the gene transfer techniques, cell culture techniques and techniques for the characterization of the efficiency of the methodologies
Place(s) where the thesis work will be carried out: University of Ljubljana
Additional information
Maximum number of students: 1